Research Associates (Bioprocess development) at Yoh in Cambridge, Massachusetts

Job Description:

BIOPROCESS ASSOCIATE (UPSTREAM, DOWNSTREAM PROCESSING, CGMP, STERILE MANUFACTURING)
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SUMMARY:

* Hiring two (2) Bioprocess Research Associates who will be working on Reagent production (Vector Core) for research, participate in Process Development (PD) activities - Bioprocess support, sterile manufacturing, media prep, Reagents, PD, Upstream and Downstream Processing; with excellent interpersonal skills.

* BS/MS with 1- 5+ years of sterile manufacturing, Gene Therapy, or R&D (including Molecular Biology techniques, Cell Based Assays, Immunoassays, Protein Biochemistry and Assay optimization along with technical writing and presentation skills.

RESPONSIBILITIES:

* Main goal within this company is to generate assays and reagents to support efforts of building a proprietary nucleic acid therapy platform with diverse tropisms and therapeutic modalities.

* Assist in performing various assays like transfections, Western Blots, qPCRs, Flow Cytometry, and Protein Expression studies in cell lines.

* Supporting experiments for production of virus particles using recombinant DNA methods and its characterization using molecular biology and virology techniques.

* Assist with experiments to look for replication and packaging of viral particles in various cell lines.

* Assist in maintaining general laboratory functionality, including lab equipment maintenance, and ordering and receiving of lab supplies.

QUALIFICATIONS:

* BS or MS in bioengineering, chemical engineering, biology, virology, biochemistry or related

* 1-5 yrs experience in the Biotech industry experience and knowledge of cGMP, sterile manufacturing, Fermentation, Mammalian Cell Culture, Bioprocess Research, Molecular Cloning, ELISA, Cell Biology, Molecular Biology, Western Blotting, Polymerase Chain Reaction (PCR), Protein Purification, SDS-PAGE, Plasmid Preparation, Buffer Preparation, Media Prep, aseptic techniques and proper growth and maintenance of cell lines.

* Demonstrated ability in working with highly skilled teams in a fast-paced, entrepreneurial and technical environment.

* Expertise in protein and gene therapy vector purification, process scale-up, and manufacturing

* Background in Cell Biology, Biochemistry, and (Bio) Chemical engineering will be needed.

ADDITIONAL EXPERIENCE DESIRED:

* Research and Develop clarification, Tangential flow filtration (TFF), exonuclease digestion, column chromatography purification methods

* High throughput methods; plasmid purification method and/ or AKTA skid method to generate enough pre-clinical and clinical material

* Cell lysis and neutralization methods to remove more impurities to achieve high performance for TFF and chromatography processing.

* Evaluate purity test results such as qPCR, HPLC, agarose DNA gel assay, provide feedback to analytical group, tech transfer of in-house downstream methods.

Key Skills:

Biotechnology, Biomanufacturing I and II Lab, Buffer & Media Preparation, Protein Purification, Technical Writing for Biomanufacturing,

Laboratory Safety, Lean Manufacturing/ Biomanufacturing, Aseptic Technique, cGMP, Bioprocessing, viral vectors, Cell Culture, Biosafety Cabinet , Quality Control (Environmental Monitoring), Bioreactor, GMP/cGMP, SOPs, FDA regulations (21 CFR 210/211), Cell culture (Bacterial and Mammalian) Upstream Processing, Downstream Processing, Bioreactors, Fermentation, Centrifugation, ELISA, PCR, Western Blot, SDS-PAGE, Media Prep, Buffer Prep, Reagent prep, Agarose Gel Electrophoresis / UV/VIS Spectrophotometry, Protein Purification / Buffer & Media Prep /Centrifugation, TFF UF/DF, Chromatography AKTA Pure System (Ion Exchange, Affinity, Size Exclusion and Hydrophobic, Cell Counting (automated and manual using Hemocytometer), Writing SOPs, using Batch Records and SOPs for upstream and downstream processing, Validation and calibration of equipment (Autoclave, Pipette, scale and pH meter), Lab Projects, Production of antibody using CHO cells, Production of myoglobin using E-coli, Bacterial transformation to produce Green Fluorescent Protein.

PLEASE FEEL FREE TO REACH ME AT 617-517-0920/REKHURAM.CHHETRI@YOH.COM.

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Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer, M/F/D/V. Click here:
http://www.yoh.com/applicants-with-disabilities
to contact us if you are an individual with a disability and require accommodation in the application process.