Associate Scientist II, E. coli Culture at ALDEVRON MADISON, LLC in Madison, Wisconsin

Job Description:

The Associate Scientist II, E. coli Culture is responsible for all duties relating to expression of recombinant proteins in bacterial shake-flask cultures. Additionally, this individual is responsible for management of the E. coli culturing and processing rooms. The Associate Scientist II must interact closely with members of the purification team to establish a culturing schedule and to ensure that cultures are completed per specifications. The person in this role may also support E. coli fermentation, eukaryotic expression, protein purification and various other lab support roles as assigned.

Responsibilities

The Associate Scientist II is expected to have good working knowledge of molecular biology and microbiology and is accountable for the responsibilities below:

• Complete all laboratory work, including execution, analysis and reporting of E. coli culturing function and other manufacturing activities as needed


• Maintain the E. coli culture and processing rooms


• Prepare microbiological culture media and other culture reagents


• Manage inventory of culture related raw materials


• Transform  E. coli with plasmid DNA


• Express recombinant protein in E. coli or mammalian cells lines


• Harvest E. coli cultures using centrifuges and TFF devices


• Use and maintain laboratory equipment including, but not limited to, environmental shakers, centrifuges, biosafety cabinets, spectrophotometers, pipettes, tangential flow filtration, autoclave and pH meter


• Maintain meticulous record keeping


• Prepare and analyze cell lysates (SDS PAGE, Western Blot, Concentration Assays)

Required Skills

• Knowledge of documentation protocol


• Good communication skills


• Good working knowledge of Microsoft Office


• Ability to manage time and multiple priorities to meet deadlines


• Ability to work independently and as part of a team

Must-Haves

• A.S. in microbiology, molecular biology, biochemistry or related field required; B.S. preferred


• 1 or more years’ prior lab experience, preferably with E. coli and protein purification

Work Hours/Shift

Monday-Friday – 8 am – 5 pm

Join Us Today!

Join a team committed to making lives better. Aldevron provides high-quality plasmid DNA, proteins, enzymes, antibodies, and other biologics to help our partners achieve significant breakthroughs for a variety of life science applications.

If you would like to thank someone for referring you to this role, please apply at www.aldevron.com/careers, and include their first and last name as a referral source!

Aldevron is committed to equal opportunity for all employees and applicants. We are committed to creating a diverse workforce and consider all qualified applicants without regard to race, religion, color, sex, national origin, age, sexual orientation, gender identify, or protected veteran status among other factors.

 

 

Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities

The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information. 41 CFR 60-1.35(c)

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