EEO Statement We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions, or any other characteristic protected by law.
Research Assistant II Lab Harvard T.H. Chan School of Public Health 58761BR
The Department of Immunology and Infectious Disease (IID) is a dynamic group of laboratories that work on the biological, immunological, epidemiological, and ecological aspects of viral, bacterial, and protozoan infections of humans, including the vectors that transmit these infectious agents. Research in many of the laboratories within the department is focused on diseases of developing countries.
As part of the infectious disease initiative, the Research Assistant (RA) will assist in research aimed at leveraging population genetics of human malaria, Plasmodium falciparum, toward biological understanding. Biological investigation of genetic loci that may play a role in parasite survival (i.e., drug resistance loci) using gene editing approaches is anticipated.Position Description
Duties and responsibilities include, but are not limited to, the following:
Tissue culture of P. falciparum, including preparation of media and culture reagents, long-term storage and retrieval of cell lines
Culture adaptation of patient derived samples and sub-cloning, drug testing and other phenotypic assays, and isolation of nucleic acids (DNA and RNA) and protein samples
Execute basic molecular biology techniques including gene cloning, transfection, and analysis of DNA, RNA and protein using standard laboratory techniques
Carry out gene-editing activities using CRISPR or other approaches
Molecular and genotyping studies that utilize polymerase chain reaction (PCR), real-time PCR, whole genome amplification, gene cloning and use of a number of genotyping platforms including but not limited to Taqman and High-Resolution Melt assays
Carry out selective whole genome amplification and library construction for whole genome sequencing
Support any number of other routine molecular biological procedures as well as basic laboratory duties
Performs other duties as assigned
The candidate will be expected to manage samples and data with precision, and perform simple and routine computational activities to summarize biological data. The candidate is required to interact with a large variety of other staff and researchers including graduate students, postdoctoral fellows and senior scientists, as required for each responsibility. The candidate will be directly supervised in the laboratory and report to a Research Scientist in the lab, as well present their work in group and laboratory meetings to update the Principal Investigator.
PLEASE NOTE: This is a grant-funded term appointment through 06/30/2023, with the possibility of extension.
PLEASE NOTE: Finalists will be required to complete an occupational health screening.
PLEASE NOTE: The primary work location for this position is Boston, MA and in order to be on Harvard payroll, you must reside in one of the following states - MA, NH, RI, ME, CT, VT, NY, or MD. Until our return to campus and full operational status, which is still to-be-determined, this interview process and position will take place in a slightly modified manner - including virtual interviews and a combination of remote and on-site onboarding and work. On-campus/in-person work is expected, though, upon start date - in accordance with approved COVID-related guidelines and protocols.
2+ years of related experience required; a combination of education and experience may be considered
Additional Qualifications and Skills
The following job-specific skills and competencies are preferred:
Knowledge of molecular biology methods and tissue culture
College coursework in Biology or related field
Experience with P. falciparum a strong plus
Experience working with computers
Strong analytical and quantitative skills
High level of initiative and the works well with little supervision
Excellent organizational skills, attention to detail, communication skills, and can effectively multi-task
Good interpersonal skills and works well in a team environment
The following cultural competencies are also preferred:
Awareness of and aptitude to appropriately and effectively understand, respect, and adapt to cultural and identity-based difference within group environments
Knowledge of and commitment to concepts and issues tied to social justice, diversity, equity, and inclusion
Skills related to creating and supporting an environment that allows for inclusion, effective intercultural engagement, and personal humility and authenticity
Commitment to Equity, Diversity, Inclusion, and Belonging
At the Harvard T.H. Chan School of Public Health, we believe that diversity is integral to the Harvard experience and our mission of improving public health education, research and policy. Diversity of cultural backgrounds, identities, lived experiences, perspectives, and ways of understanding the world enriches our community and enables us to best meet the public health needs of the United States and the world. Ongoing learning and development related to diversity allows for both individual and institutional growth, and is necessary to foster and sustain a culture of inclusion. To achieve this, we are committed to ensuring equitable access to opportunities for learning, living, and working at the Harvard Chan School. We maintain an unwavering dedication to diversity, inclusion, and belonging as core to our institutional values and to actively counter, minimize, and address racism, sexism, xenophobia, homophobia, gender bias, and all forms of discrimination.
Learn more about the School's mission, objectives, and core values and our diversity, inclusion, and belonging initiatives.