Research Assistant I - Genomic Analysis Laboratory, Dr. Ecker at Salk Institute for Biological Studies in La Jolla, California

Job Description:

The Research Assistant I position will be working as part of a team with the goal of using epigenomics to create an atlas of the human brain at single-cell resolution, through both sequencing and imaging.  This position will involve training in specialized techniques related to the Ecker lab focus of single-cell multiomics.  Main duties for this position may include: large-scale generation of methylation sequencing libraries using a robotic pipetting system, Cryostat sectioning and fluorescence imaging for spatial transcriptomics through MERSCOPE, nuclei production and sorting, and treatment of nuclei to evaluate chromatin conformation.  Other duties include general laboratory maintenance, coordination with collaborators and FACS core for cell sorting, participation in regular team meetings to discuss progress, and well-organized LIMS and data recording.

ESSENTIAL FUNCTIONS

(90%) Large-Scale Molecular Biology Techniques

• Bisulfite sequencing


• Magnetic bead DNA purification


• PCR


• Perform tissue sectioning with Cryostat


• Fluorescence staining protocols for imaging


• Isolate cellular nuclei from brain tissue


• Chromatin assays

(10%) Lab maintenance and organization

• Sample tracking and scheduling


• Coordination with others for sample processing


• Updating LIMS as necessary with sample information


• Attend weekly lab meetings and assist with problem solving


• General lab/equipment upkeep

EXPERIENCE

Required:

• No professional experience required with relevant degree

Preferred:

• 0-2 years of experience as a lab technician or equivalent related experience.


• Experience with robotic liquid handling like Biomek or Tecan


• Familiar with molecular biology techniques such as PCR, next-generation sequencing (NGS) library preps, chromatin assays, ATAC-seq, bisulfite sequencing

EDUCATION

Required:

• Bachelor's degree in Biological Sciences or related scientific discipline required. Bachelor's degrees in a different discipline and a minimum of 2 years of experience in a scientific role will be considered in lieu.

Preferred:

• None

SKILLS AND ABILITIES

Required:

• Theoretical and hands-on knowledge of molecular biology techniques.


• Proven ability to work both independently and also as part of a collaborative team.


• Ability to learn and carry out new experimental procedures with accuracy and efficiency. Demonstrated ability to follow verbal and/or written protocols, including follow-up on previously begun procedures.


• Demonstrated ability to record data accurately and enter into appropriate medium.


• Skill in the operation and maintenance of various lab equipment.


• Highly organized, effective at multitasking, adept to changing priorities


• Demonstrated knowledge and experience with both PC and MAC operating systems, standard business office equipment and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.


• Proficiency with common office software applications as well as software applications used for research, recording, and analysis.


• Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions.


• Excellent communication skills, both oral and written.


• Strong interpersonal skills, including tact, diplomacy, and flexibility.


• Demonstrated ability to interact effectively with diverse groups. Demonstrated skill in establishing and maintaining effective working relationships with personnel at all levels.

Preferred
:

• None

The salary range for this position is $18.02 - $23.91 hourly.

SPECIAL CONDITIONS OF EMPLOYMENT

• Must be willing to work in a human/animal-related research environment.


• Must be willing to work a flexible schedule to support research needs.


• Successful completion of the Institute’s background investigation.

PHYSICAL REQUIREMENTS/MENTAL ACTIVITIES/ENVIRONMENTAL CONDITIONS

The incumbent in this position will be constantly adjusting focus, grasping, hearing, seeing, talking, analyzing, calculating, communicating, reading, writing, and working indoors.