Posted in Other 30+ days ago.
Type: Full Time
GENERAL SUMMARY/OVERVIEW STATEMENT:
Working under the general supervision of the Principal Investigator, and in accordance with established hospital policies and procedures, executes experiments involving in vitro and in vivo protocols and advanced molecular techniques. Participates in preclinical studies focused on the identification of cancer biomarkers and testing of novel targeted therapies in murine models and human tumor xenografts. May participate in the orientation and training of new laboratory staff and interns.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Under general supervision, conducts molecular, cell biology, and immunology procedures, such as DNA extraction, conventional PCR and real time PCR, dot blot analysis, IHC, immunofluorescence, western blotting, FACS, MTT assays, and development and optimization of 3D ex vivo organotypic models.
Maintains tissue cultures and manipulates tumor cells and lines in a variety of experimental conditions. Monitors cells/cultures for viability and contamination. Perform cell viability MTT assays using various chemotherapy compounds and molecularly targeted therapies. Performs lentiviral and retroviral infection, plasmid transfection, and immunoblotting of tumor cells and controls.
Performs animal work, including iv and ip injections, maintenance and genotyping of animals, and surgical procedures, such as intrabursal, intra-uterine, and intra-fallopian tube of tumor lines or Adeno-Cre injections.
Calculates and analyzes data using statistical techniques and contributes to the writing of manuscript text and figures submitted for publication and grant applications.
Discusses progress of work regularly with Principal Investigator.
Maintains tissue culture and molecular biology supplies and reagents and helps order new supplies and minor equipment.
Assists with coordinating laboratory compliance with institutional policies and procedures in the areas of safety, environmental, and infection control.
Assists in the orientation and training of new laboratory staff, COOP students, and interns if necessary.
Performs all other duties and responsibilities as directed, including both research and administrative duties.
Bachelor of Science or Bachelor of Arts in a biological science.
New graduate with relevant course or project work.
Sound analytical and organizational skills.
High degree of computer literacy.
Careful attention to detail.
Highly motivated and able to performs experiments independently once trained.
Able to conduct in vitro and in vivo experiments.
Excellent oral and written communication skills.
Must have sound interpersonal skills.
Normal basic science laboratory environment.
Exposure to laboratory reagents, chemicals, adenoviruses, retroviruses, lentiviruses, and patient and murine tumor cells under controlled conditions. Minimal risk when established protocols are followed.
Will participate in the orientation of new research staff if necessary.
Brigham and Women's Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, sex, color, religion, national origin, sexual orientation, protected veteran status, or on the basis of disability.