Research Technician I at Seattle Children's Hospital in Seattle, Washington

Job Description:

About Us
Hope. Care. Cure. These three simple words capture what we do at Seattle Children's - to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.

Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine.

Researchers work in close collaboration with one another, their colleagues at partner institutions including the and and our healthcare providers at Seattle Children's Hospital, one of top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.

We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.
Overview
Perform laboratory procedures and experiments investigating: 1) the role of airway epithelial cells in pediatric asthma, and 2) host airway epithelial innate immune responses to viral infections, including human rhinovirus and SARS-CoV2 infection. Preferred Technical skills include human primary airway epithelial cell, lung stromal cell, and immune cell tissue culture, differentiation of airway epithelial cells at an air-liquid interface, gene knockdown using lentiviral shRNA and gene editing using CRISPR/Cas9 methods in primary human airway epithelial cell lines, microscopy (phase contrast; fluorescent), biochemical assays, RNA isolation, qPCR, protein assays and ELISA. SARS-CoV2 work includes infection of airway epithelial cells in a BSL-3 laboratory.
Work as part of a team with lab technicians, students, clinical research associate, and principal investigators to complete daily lab tasks, Collect, verify and record data; compile information in support of research. Apply knowledge and skills applicable to assigned technical discipline.
Requirements
Required Education/Experience:
- Bachelor's Degree in an appropriate field of science or technology OR college-level coursework in biology, molecular biology, biochemistry, microbiology, or in a related science curriculum AND equivalent lab experience.

Preferred:
Prior Lab Experience with:
- Set up and maintenance of tissue culture
- RNA isolation, cDNA synthesis, quantitative PCR using TaqMan probes
- Conduct of protein and ELISA assays
- Use of shRNA or siRNA for gene knockdown experiments and/or CRISPR-Cas9 gene editing in primary cells
- Flow cytometry
- Experience with phase contrast and fluorescent microscopy
Our Commitment to Diversity
Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

The people who work at Seattle Children's are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.

Seattle Children's is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.

- Area of Interest: Research & Development;
- FTE/Hours per pay period: 1.0 FTE (80 hours per bi-weekly pay periods);
- Work Status: Regular;
- Department: Immunity & Immunotherapies;
- Shift: Day Shift;
- Job ID: 37004;.