Exciting opportunity for motivated research technician to join a translational laboratory studying various aspects of cellular adaptive immunity, especially in relation to T regulatory cell characterization, function and expansion, as well as the effects of various immune manipulations. We have a history of successfully conducting basic and preclinical experiments on new biologic agents and translating them into the clinic, and currently collaborate in clinical trials studying the adaptive use of T regulatory cells in transplant settings and in cancer.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
This position will assist scientists in performing scientific research, development and/or clinical tasks of varying complexity. In general, this position will assist in the set up, operation and maintenance of laboratory instruments and equipment, monitoring experiments, making observations and calculating and recording results.
A successful candidate will have the following characteristics:
Reliable, organized, and flexible with ability to handle multiple activities and to reprioritize tasks
Understands lab etiquette and participation in general laboratory responsibilities (ordering and organizing laboratory supplies and reagents)
Able to work with other team members and collaborators as well as complete independent work
Ability to analyze information and use logic to address work-related issues and problems
Must have attention to detail and be thorough in completing work tasks
Must have excellent communication skills (written and verbal) and be able to work within a team environment with a wide variety of faculty and staff
Able to work closely with supervisor and staff scientists to analyze data and interpret results of experiments within the context of research project goals, and participate in or take responsibility for SOP generation
Set up, adjust, calibrate, clean, maintain and troubleshoot equipment.
Clean, maintain and prepare supplies and work areas and may be responsible for ordering of supplies. Collaborates with Purchasing and may be responsible for negotiating with vendors.
Conduct standardized biological, microbiological or biochemical tests and laboratory analyses, including the collection of information and samples from organic substances, such as blood, drugs, and animals.
Isolate, identify and prepare specimens for examination.
Analyze experimental data and interpret results to write reports and summaries of findings, including grant preparation and presentations, and look for opportunities to co-author publications. May be expected to participate in formal and information presentations of results to staff.
Participate in grant application process and manuscript preparation as required.
Maintains detailed notebook of all work-related activities.
Monitors laboratory work to ensure compliance with Environmental Health and Safety information as well as the standard operation procedures specific to each laboratory.
Expected to provide intellectual and interactive commitment to position by keeping up with appropriate literature and research publications.
May be responsible for the development, modification and improvement of standard operating procedures.
Uses computers, computer-interfaced equipment, robotics or high-technology applications to perform work duties.
May be responsible for designing and executing advanced experiments and setting strategy.
May be responsible for the examination of animals and specimens to detect the presence of disease or other problems.
May be responsible for monitoring laboratory budgets.
Relevant experience in academia or industry strongly preferred. Bachelor's degree with some experience and desire to learn considered. Coursework in related fields (such as Immunology and Molecular Biology) a plus
Preference given to candidates with human cellular immunology experience, for example isolation of peripheral blood cells for characterization, purification, and cryopreservation; cell culture; sterile technique; cell-based assays including mixed lymphocyte culture and use of various molecular biology and immunology techniques to assess immune responses. Prior experience with T cell immunology also a strong plus
Flow cytometry experience highly desirable
Experience in analyzing biological data using GraphPad Prism and Excel required. Experience with FlowJo and SoftMax Pro and any array analysis experience strong positives
Other relevant experience includes multianalyte analysis (e.g. Luminex), ELISA, DNA and RNA isolation, PCR and RT-PCR, genechip and microarray, assay development and adaptation
Willingness to make at least a 2 year commitment
Bachelor's Degree required with a major in biology or chemistry preferred.
Knowledge of information and techniques related to either biology, chemistry, or medicine is preferred.
Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
Ability to analyze information and use logic to address work-related issues and problems.
Must have attention to detail and be thorough in completing work tasks.
Ability to work within a team environment.
Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.