Research Assistant III- Blood Brain Barrier at Harvard University

Posted in Research 26 days ago.

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Location: Boston, Massachusetts

Job Description:

EEO Statement
We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions, or any other characteristic protected by law.

Duties & Responsibilities
The Wyss Institute for Biologically Inspired Engineering at Harvard University seeks an enthusiastic individual to work as a Research Assistant in the Bioinspired Therapeutics & Diagnostics platform. The successful candidate will significantly contribute to a research program involving the use of cell culture and microfluidic in vitro models to support our efforts in novel drug discovery and antibody delivery across the blood-brain barrier (BBB). The successful candidate will be responsible for the optimization and execution of microfluidic BBB-on-Chip primary cell culture and the development and validation of various in-vitro and cell-based assays, including, but not limited to cell-based qPCR/TaqMan, ELISA, westerns, enzymatic assays, imaging, and flow cytometry. Previous experience with mammalian cell culture is a must, while previous knowledge and/or experience with endothelial in vitro or in vivo models, as well as endothelial development and IPS technology, is a plus. The candidate will be expected to work independently under the supervision of staff and faculty to design experiments, learn new skills, troubleshoot, and to contribute intellectually to the project. In addition, the candidate is expected to order supplies, perform support activities and lab maintenance, and function in a team-oriented environment with other scientists and staff at the Institute as well as external collaborators. The candidate will participate in the preparation of manuscripts and patent applications. Under the supervision of staff, the candidate will present data and conclusions at meetings within the Wyss Institute.

The mission of the Wyss Institute for Biologically Inspired Engineering at Harvard University is to transform human healthcare and the environment by emulating the way nature builds. Developed as an alliance between Harvard and other premier academic and clinical partner institutions, Institute faculty and staff collaborate in high-risk, fundamental research and science-driven technology development. A major focus of the Institute is to translate the technologies developed by its faculty and staff into commercial products and therapies through collaborations with clinical investigators and establishment of corporate alliances and launching startup companies. For more information, visit:

Harvard offers an outstanding benefits package including:
  • Time Off: 3 - 4 weeks paid vacation, paid holiday break, 12 paid sick days, 11.5 paid holidays, and 3 paid personal days per year.
  • Medical/Dental/Vision: We offer a variety of excellent medical plans, dental & vision plans, all coverage begins as of your start date.
  • Retirement: University-funded retirement plan contributions from 5% to 15% of eligible compensation, based on age and earnings with full vesting after 3 years of service.
  • Tuition Assistance Program: Competitive tuition assistance program, $40 per class at the Harvard Extension School and discounted options through participating Harvard grad schools.
  • Transportation: Harvard offers a 50% discounted MBTA pass as well as additional options to assist employees in their daily commute.
  • Wellness options: Harvard offers programs and classes at little or no cost, including stress management, massages, nutrition, meditation and complimentary health services.
  • Harvard access to athletic facilities, libraries, campus events and many discounts throughout metro Boston.

Basic Qualifications
Bachelor's degree in biology, molecular biology, biochemistry, bioengineering, or related field. At least two years of experience involving aseptic mammalian cell culture and molecular and cell biology techniques, such as ELISA, PCR, microscopy, PCR, Western blots, Fluorescence-activated cell sorting (FACS) or related experience. Advanced degree may count towards years of experience.
Additional Qualifications
Experience with primary or iPSC-derived cells, including brain endothelium. Experience with cell culture in microfluidic device is a plus; experience with antibody research is a plus. Ability to work well independently and to collaborate with a multidisciplinary group is essential. The candidate should be proficient with personal computers and applicable software packages (Microsoft Office). Good written and oral communication skills are essential. The candidate should be highly motivated, detail-oriented, organized, and able to take initiative.